Mouse Cell Line Authentication

For human cell lines short tandem repeat str loci are one of the most informative polymorphic markers in the human genome. It s a comprehensive cell line authentication service that utilizes str based dna profiling and multiplex pcr to detect both contamination and misidentification of your cell lines. However few assays are available for nonhuman cell line identification.

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An organized mouse cell line authentication consortium in partnership with nist was created.

Mouse cell line authentication. Identity verification with str analysis dna fingerprinting for mouse cell lines while human cell line authentication has been addressed with a short tandem repeat str profiling up until now there has not been a validated method for identifying mouse cell lines beyond the species level. Almeida jl hill cr cole kd 2011 authentication of african green monkey cell lines using human short tandem repeat markers. Sign up for our quarterly enewsletter for the latest cell line authentication news and tips. We have developed a multiplex polymerase chain reaction.

The consortium for mouse cell line authentication was formed to validate short tandem repeat str markers for intraspecies identification of mouse cell lines. Ecacc offer cell line authentication services for both human and animal cell lines. The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells. Mouse cell line authentication str profiling includes two human markers to pick up cross contamination.

Almeida jl hill cr cole kd 2013 mouse cell line authentication. Mouse cell line authentication. We have developed a multiplex polymerase chain reaction assay that targets nine tetranucleotide short tandem repeat str markers in the mouse genome. Cooperative research and development agreement crada are in place between nist and consortium members.

Dna barcoding can distinguish between species but not between individual animals of the same species. The str profiling method is a. An easy and comprehensive service we accept your cryovial and there is no need for ordering special sample collection supplies. In order to detect cell line contaminants primers for two human str markers were incorporated into the multiplex assay to facilitate detection of human and african green monkey dna.

Atcc now provides a powerful str profiling service that authenticates mouse cell lines and detects. Labcorp offers g banded human karyotyping services for stem cell lines and more. Each crada is identical so that every laboratory is working under the exact same conditions in the agreement and is protected equally. The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells.

However there are few assays available for nonhuman cell line identification. This multiplex assay is the first of its kind to provide a unique str profile for each individual mouse sample and can be used to authenticate mouse cell lines.